目的 探究光热与基因联合治疗递送系统后续的酶降解过程。方法 用肽脂质包覆碳纳米管构建光热/基因联合治疗递送载体单壁碳纳米管-肽脂质复合载体(CDO/SCNT)和多壁碳纳米管-肽脂质复合载体(CDO/MCNT)。采用热重分析考察递送载体的结合程度。使用辣根过氧化物酶(HRP)体外模拟酶环境,通过拉曼光谱和透射电子显微镜观察递送载体的酶降解性能。结果 CDO/SCNT中SCNT占38.5%,CDO/MCNT中MCNT占34.2%,肽脂质成功包覆在碳纳米管表面。载体在酶环境下7 d就发生降解,28 d时,拉曼光谱可见CDO/SCNT载体D带的结晶结构紊乱程度与G带的E2g振动信号峰强度比值达到0.48,而未包覆肽脂质层的SCNT达到0.31;透射电镜观察到2种递送载体中碳纳米管破碎;CDO/SCNT的酶降解程度要高于CDO/MCNT。结论 HRP酶可催化H2O2氧化石墨结构生成CO2,肽脂质层有助于碳纳米管(CNTs)的酶降解,CDO/SCNT载体具有良好的酶降解性能。
Abstract
OBJECTIVE To explore the enzyme degradation mechanism of the combined therapy delivery system of photothermal and gene therapy. METHODS Herein, cationic lipids were used to coat single-walled carbon nanotubes (SCNT) and multi-walled carbon nanotubes (MCNT) to form delivery systems (denoted CDO/SCNT and CDO/MCNT). The results of thermos gravimetric analysis showed that the lipid layer was successfully coated on the surface of carbon nanotubes. Horseradish peroxidase (HRP) was used to simulate the enzyme environment in vitro to investigate the enzyme degradation performance of the delivery carrier. RESULTS The integrity of the graphite structure in the carbon nanotubes was observed by Raman spectroscopy and TEM on day 0, with the extension of degradation time, the degradation degree of carbon nanotubes gradually increased within 28 days. The ratio of the disordered crystal structure of the D band of CDO/SCNT carrier to the E2g vibration signal intensity of the G band reached 0.48, while the SCNT of the uncoated lipid layer reached 0.31. The fragmentation of carbon nanotubes boththe two delivery carrier was also observed using TEM, and the degree of enzyme degradation of CDO/SCNT delivery carrier was higher than that of CDO/MCNT. CONCLUSION Thus, the HRP can catalyze the degradation of carbon nanotubes by H2O2, oxidize the graphite structure to form CO2, and the peptide lipid layer contributes to the enzymatic degradation of CNTs.
关键词
碳纳米管 /
肽脂质 /
递送系统 /
酶降解
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Key words
carbon nanotube /
peptide lipid /
delivery system /
enzymatic degradation
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中图分类号：
R944
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脚注
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基金
国家自然科学基金项目资助(21776044;21606041);校级大学生创新创业训练计划项目资助(202112026214;202112026168)
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